By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra info for Advances in lipid research. Volume 6
The following year Nikkilä (1953) and Swahn (1953) published extensive studies of healthy and diseased human subjects. Important information on technique was published by Langan et al. (1955) and Jencks and Durrum (1955). Addition of albumin or lipoprotein-poor serum to either the lipoprotein samples or to the electrophoresis buffer gave better patterns than had b e e n obtained previously (Smith, 1957). Further modifications of lipoprotein electrophoresis were reported by Lees and Hatch (1963), who achieved higher resolution by addition of albumin to the buffer and other changes.
891 at 37°C. Plasma Lipoprotein 6. 41 Analysis Care ofElectrophoresis Cell An important part of obtaining reproducible lipoprotein electrophoresis is the routine maintenance of the electrophoresis cell. If runs are to occur daily the cell may be kept at room temperature —progressive growth of bacteria or molds has not b e e n noted. For longer intervals it should be stored at 4°C, allowing sufficient time for rewarming before the next run. T h e central partition and the racks should b e cleaned of buffer deposits and dried b e t w e e n runs.
A quantity of lipoprotein containing 5 — 20 mg of protein, as measured by the method of Lowry et al. (1951) in about 1 ml of saline or barbital buffer is emulsified with an equal volume of Freund's complete adjuvant (Difco Laboratories, Detroit, Michigan). This is most easily done by placing the lipoprotein and the adjuvant in separate 5ml Luer-Lock syringes with 20-gauge needles and connecting the two needles via a 1-in. 030 in. d. polyethylene tubing. 21 ß sf sf Sf 0-400 /3 + Pre-/3 "Three populations — 77 cases.
Advances in lipid research. Volume 6 by Rodolfo Paoletti, Dr. David Kritchevsky